Masami Yoshimura

Masami Yoshimura

Associate Professor

Department of Comparative Biomedical Sciences

LSU School of Veterinary Medicine
Louisiana State University
Baton Rouge, LA 70803

EMAIL DR. YOSHIMURA

Education

DSc, Kyoto University (Japan)

MSc, Kyoto University (Japan)

BSc, Kyoto University (Japan)

Research Interest

Physiological effects of alcohol drinking via cyclic AMP signaling system. Current research is to elucidate the role of adenylyl cyclase type 7 and alcohol drinking in innate immune responses of the lung. 

Teaching Interest

Cell and molecular biology, pharmacology

Awards & Honors

2012-2021 Dean’s Teacher Merit Honor Roll

2010  Pfizer Award for Research Excellence 

Publications

Yoshimura, M., Pearson, S., Kadota, Y. and Gonzalez, C.E. (2006) Identification of ethanol responsive domains of adenylyl cyclase. Alcohol. Clin. Exp. Res . 30, 1824-1832. https://pubmed.ncbi.nlm.nih.gov/17067346/

Hines, L.M., Hoffman, P.L., Bhave, S., Saba, L., Kaiser, A., Snell, L., Goncharov, I., LeGault, L., Dongier, M., Grant, B., Pronko, S., Martinez, L., Yoshimura, M. and Tabakoff, T.; WHO/ISBRA Study on State and Trait Markers of Alcohol Use and Dependence Investigators. (2006) A sex-specific role of type VII adenylyl cyclase in depression. J. Neurosci. 26, 12609-12619. https://pubmed.ncbi.nlm.nih.gov/17135423/

Kou, J. and Yoshimura, M. Isoform specific enhancement of adenylyl cyclase activity by n-alkanols. (2007) Alcohol. Clin. Exp. Res .31, 1467-1472. https://pubmed.ncbi.nlm.nih.gov/17760784/

Subach, O.M., Gundorov, I.S., Yoshimura, M., Subach, F.V., Zhang, J., Grüenwald, D., Souslova, E.A., Chudakov, D.M. and Verkhusha, V.V. (2008) Conversion of red fluorescent protein into a bright blue probe. Chem. Biol. 15, 1116-1124. https://pubmed.ncbi.nlm.nih.gov/18940671/

Hasanuzzaman, M. and Yoshimura, M. (2010) Effects of straight chain alcohols on specific isoforms of adenylyl cyclase. Alcohol. Clin. Exp. Res. 34, 743-749. https://pubmed.ncbi.nlm.nih.gov/20102569/

Grammatopoulos, T.N., Jones, S.M., Yoshimura, M., Hoover, B.R., Das, M., Snyder, E.Y., Larson, G.A., Zahniser, N.R., Tabakoff, B., Zawada, W.M. (2010) Neurotransplantation of stem cells genetically modified to express human dopamine transporter reduces alcohol consumption. Stem Cell Res Ther 1, 36. https://pubmed.ncbi.nlm.nih.gov/21122109/

Dokphrom, U., Qualls-Creekmore, E. and Yoshimura, M. (2011) Effects of alcohols on recombinant adenylyl cyclase type 7 expressed in bacteria. Alcohol. Clin. Exp. Res. 35, 1915-1922. https://pubmed.ncbi.nlm.nih.gov/21635274/

Gupta, R., Qualls-Creekmore, E. and Yoshimura, M. (2013) Real-time monitoring of intracellular cAMP during acute ethanol exposure.  Alcohol. Clin. Exp. Res. 37, 1456-1465. https://pubmed.ncbi.nlm.nih.gov/23731206/ 
Hill, R.A., Xu, W. and Yoshimura, M. (2016) Role of adenylyl cyclase isoform in ethanol’s effect on cAMP regulated gene expression in NIH 3T3 cells. Biochem. Biophys. Rep. 8, 162-167. https://pubmed.ncbi.nlm.nih.gov/28620651/

Qualls-Creekmore, E., Gupta, R. and Yoshimura, M. (2017) The effect of alcohol on recombinant proteins derived from mammalian adenylyl cyclase. Biochem. Biophys. Rep. 10,157-164. https://pubmed.ncbi.nlm.nih.gov/28955743/  

Grants

R03AA029165 Yoshimura (PI), 9/25/2021-8/31-2023, NIH/NIAAA, Role of AC7 and alcohol in innate immune responses during bacterial infection. The major goal of this pilot study application is to examine the acute effect of ethanol on the innate immune response of myeloid cell lineage-specific AC7 KO mice.

CORP grant Yoshimura (PI), 7/1/2021-6/30/2022, LSU Vet Med, Suppression of lung innate immune responses by acute alcohol exposure: role of adenylyl cyclase type 7. The major goal of this pilot study application is to examine the acute effect of ethanol on the innate immune response of myeloid cell lineage-specific AC7 KO mice and generate preliminary data for extramural grant application. 

Department Research Fund (PI), 11/1/2019-6/30/2020, LSU Vet Med, The major goal of this pilot study is to generate preliminary data for the effect of ethanol on AC7 expression in mouse and human alveolar macrophages using RNA scope technology.

COBRE pilot Yoshimura (PI), 7/1/2019-12/31/2019, LSU/Center for Lung Biology and Disease, The major goal of this pilot study is to generate preliminary data for innate immune responses in acute lung injury induced by LPS and alcohol using myeloid lineage specific AC7 KO mice.

Department Research Fund (PI), 7/1/2017-6/30/2019, LSU Vet Med, The major goal of this pilot study is to generate preliminary data for the effect of ethanol on innate immune response of AC7 KO myeloid cells and mice.

CORP grant Yoshimura (PI), 7/1/16-6/30/17, LSU Vet Med, Role of Type 7 Adenylyl Cyclase in Ethanol’s Effects on Immune Response. The major goal of this pilot study application is to examine the acute effect of ethanol on the innate immune response of myeloid cell lineage-specific AC7 KO mice.

LBCRP grant Yoshimura (PI), 11/1/16-4/30/18, LSU, Role of type 7 adenylyl cyclase in Innate Immunity. The major goal of this pilot study application is to examine functional alteration/defects in AC7 KO macrophages and neutrophils isolated from myeloid cell lineage-specific AC7 KO mice.

CORP grant Yoshimura (PI), 7/1/15-6/30/16, LSU Vet Med, Generation of gene knockout cell line from BV-2 microglia cell line. The major goals of this pilot study application are 1) to generate an AC7 knockout cell line from one of the immune cell lines using CRISPR Cas9 gene editing system, and 2) to confirm expected changes in cAMP signaling in the knockout cell line.  

R01 AA13148 Yoshimura (PI), 3/1/2002-4/30/2015, NIH/NIAAA. The major goals of this project are: 1) to identify a domain of AC that is responsible for the effect of ethanol on this enzyme by using a series of chimeric mutants between ethanol-sensitive and ethanol-insensitive isoforms of adenylyl cyclase (AC) and mutant AC's generated by in vitro site-directed mutagenesis and 2) to determine structural alterations induced by ethanol using NMR analysis of recombinant AC7 proteins.